Table 5.
Amino acids found at CBS-1 (P6) and CBS-2 (P8), showing that sequences of the tandem CBSs are highly conserveda
| Site | aa position in NS5A of JFH-1/H77 | aa frequency | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| CBS-1 (P6) | 308/312 | A: 97.63* | G: 0.02 | P: 0.11 | S: 2.14 | T: 0.02 | V: 0.09 | |||
| 309/313 | F: 0.04* | I: 2.00* | L: 63.78* | M: 33.90* | P: 0.07 | S: 0.02 | T 0.02 | V: 0.14* | W: 0.02* | |
| 310/314 | A: 0.02 | L: 0.07 | P: 99.77* | S: 0.14 | ||||||
| 312/316 | G: 0.02 | L: 0.02 | R: 0.16 | W: 99.80* | ||||||
| CBS-2 (P8) | 340/344 | C: 0.02 | F: 0.07* | H: 0.02 | I: 0.12* | L: 99.37* | M: 0.04* | V: 0.35* | ||
| 341/345 | A: 0.39 | G: 0.02 | H: 0.02 | P: 99.37* | S: 0.14 | |||||
a
A total of 4,307 NS5A sequences obtained from the European HCV database (EuHCVdb) were analyzed, and the numberings are of JFH-1 NS5A.
*
, residues that conform to A∅PXW of CBS-1 and ∅P of CBS-2.