Fig 7.

Neutralization patterns of HIV envelope-affinity purified, IEF-fractioned IgG from subjects with broad HIV neutralizing activity. The affinity handles used to capture IgG (see Materials and Methods) are shown at top. IEF fractions are shown on the x axis. A pH gradient of 6.5 to 9.5 spans the fractions from left to right. IgG from each fraction was tested for neutralizing activity against the indicated pseudoviruses as described in Materials and Methods. Assays were run in duplicate (except for gp120 core-purified material from subject 1). Mean infectivity values were used to calculate IC₅₀ titers (left y axis). The maximum IgG concentrations tested per fraction are shown on the right y axis. Fractions derived from subject 1 were tested as singletons due to the paucity of sample.