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The ability of A. nancymae peripheral blood lymphocytes to respond to recombinant murine IL-12 was assessed based on incorporation of [3H]Tdr into cellular DNA. Pooled lymphocytes were stimulated with dilutions of concentrated supernatants from Vero cells infected with M002 (IL-12), or with PHA as a control, and then pulsed with [3H]Tdr. Activation was assessed by scintillation count and is expressed as cpm, plotted in comparison to a linear IL-12 response.
