Fig 2.

The intergenotypic chimeric genome was fully replication competent, and the encoded polyprotein was processed efficiently. (A) Schematic diagram showing the locations of the luciferase gene and FMDV 2A in the chimeric polyproteins. (B) At 24, 48, and 72 h after the transfection of HL1 cells with GND-Luc, Jc1-Luc, JFH1-Luc, or JFH1-J4p7-Luc transcripts, intracellular luciferase activity was measured as described in Materials and Methods. (C) HL1 cells were transfected with the GND, wild-type JFH1, or JFH1-J4p7 genome. At 48 h posttransfection, the cells were lysed in 2× Laemmli sample buffer, and the viral proteins were resolved onto a 10% SDS-PAGE gel and were detected as described in Materials and Methods. Magic Marker (Invitrogen) was used as size markers.