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. 2012 May;86(9):5080–5088. doi: 10.1128/JVI.07089-11

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Fig 5 — Colocalization of p7 and NS5B. (A) Schematic representations of the constructs that were utilized to express JFH1 p7 tagged with the Flag epitope (Jp7-Flag), HCV-A p7 tagged with the Flag epitope (Hp7-Flag), JFH1 NS5B tagged with the myc epitope (myc-NS5B), and JFH1 NS5B containing K151R tagged with the myc epitope (myc-NS5BKR). (B) At 48 h after transfection of Huh7.5 cells with the constructs diagramed in Fig. 5A, cells were fixed with paraformaldehyde, and the tagged proteins were detected using antibodies to the Flag (α-Flag) and myc (α-myc) epitopes as described in Materials and Methods. Laser confocal microscopy was used to visualize the proteins in the cells. (Top) Colocalization of JFH1 p7-Flag and myc-NS5B. (Center) Colocalization of HCV-A p7 and myc-NS5B. (Bottom) Colocalization of HCV-A p7 and myc-NS5BKR. The nuclei were stained with Hoechst stain.