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Catalytic efficiencies of wild-type and mutant NS5B proteins
| Measurement (unit) | NS5B K151R |
Wild-type NS5B |
||
|---|---|---|---|---|
| Mean | SD | Mean | SD | |
| Vmax (mol/μg/h) | 1.45E−11 | 6.80E−12 | 2.97E−11 | 2.73E−11 |
| kcat (s−1) | 3.50E−04 | 1.64E−04 | 7.17E−04 | 6.60E−04 |
| Km (M) | 8.57E−06 | 9.05E−06 | 1.84E−05 | 2.08E−05 |
| kcat/Km (M−1 s−1) | 4.09E+01 | 1.81E+01 | 3.91E+01 | 3.18E+01 |
