Fig 1.

MV gp-pseudotyped LVs are highly superior to VSV-G-LVs for the transduction of immature MDDCs. (A) Day 5 immature MDDCs were transduced or not by VSV-G-LVs (middle panel) or H/F-LVs (lower panel) encoding GFP (MOI = 10). Transduction efficiencies were analyzed by flow cytometry and plotted as the percentage of GFP⁺ cells in DC-SIGN expression in immature MDDCs. (B and C) Flow cytometric analyses showing frequencies (B) and GFP MFIs (C) after the transduction of immature MDDCs with increasing doses of VSV-G-LV (empty bars) or H/F-LV (gray bars) particles (MOI = 0, 1, 2, or 10) (only MOI = 10 in panel C) (n = 5). (D) Transduction of day 5 immature MDDCs was performed as described in for panel A but with equivalent total p24 levels of VSV-G-LVs (empty bars) and H/F-LVs (gray bars) (in pg; n = 3). Error bars are indicated above each column.