Fig 7.

Encapsidation profiles and stability of recombinants during passage in N. benthamiana. (A) Northern blot analysis of 200 ng of each virion RNA preparation recovered from leaves infiltrated with the indicated agrocultures. (B) Northern blot analysis of total RNA preparations recovered at 4 dpi from N. benthamiana leaves mechanically inoculated with virion RNA preparations shown in panel A. (C) Northern blot analysis of progeny RNA recovered from uninoculated systemic leaves of N. benthamiana at 15 dpi. Conditions of Northern blot hybridization with riboprobes are as described in the Fig. 1B legend. The positions of all BMV RNAs are shown to the left, and the recombination progeny of B3R-α and B3R-β are shown to the right. (D) Schematic representation of recombinants recovered from uninoculated systemic leaves. A representative example of the nomenclature used to describe each recombinant clone is as follows: GCS, GUA, correct orientation, systemic leaves. All other features of recombinant clones are as described in the legend of Fig. 3.