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. 2012 May;86(9):5039–5054. doi: 10.1128/JVI.07162-11

Table 2.

ORFs encoded by COTV

ORFa Position (bp) Length (aa) Best matchb
 Description/putative functiond VACV-Cop orthologe
Species, gene Length (aa) % IDc
COTV001 1122-571 183 CPXV-GER1980-EP4, 205 188 32 Serine protease inhibitor-like protein C13L/C14L
COTV002 2864-1152 570 DPV-W1170-84, 019 643 25 Ankyrin repeat protein B4R
COTV003 3713-3411 100 Mustela putorius furo, gene CCL13 70 35 C-C motif chemokine protein —
COTV004 4511-3717 264 CPXV-NOR1994-MAN, 002 245 28 Chemokine binding protein C23L/B29R
COTV005 6560-4860 566 DPV-W848-83, 019 643 25 Ankyrin repeat protein/PRANC domain B4R
COTV006 7245-6613 210 Bos taurus, locus BOS-3916/CNPV-VR111, 170* 212/212 50/55 Thymidylate kinase A48R
COTV007 7777-7274 167 CPXV-UK2000-K2984, 003 355 25 TNF receptor-like protein (CrmB) C22L/B28R
COTV008 8175-7801 124 YKV, 178 127 42 TNF receptor-like protein (CrmE) —
COTV009 9230-8199 343 MPXV-ZAR-1979, 005/187 354 37 Soluble IFN-α/β receptor B19R
COTV010 9577-9245 110 Mus musculus, gene Scya2 148 25 C-C motif chemokine protein —
COTV011 10363-9581 260 CPXV-GER91, 001/219 252 26 C-C chemokine binding protein (35-kDa major secreted virus protein) B29R
COTV012 10856-10404 150 LSDV-LW1959, 001/161 159 47 NF-κB inhibitor/virulence factor B15R
COTV013 11278-10910 122 RFV-Kas, 004/163 125 49 Unknown —
COTV014 12422-11601 273 CPXV-UK2000-K2984, 009 273 31 Kelch-like protein A55R
COTV015 13562-12708 284 ECTV-Mos, 004 273 29 Kelch-like protein A55R
COTV016 15664-13919 581 MYXV-Lau, 152 675 24 Ankyrin repeat protein —
COTV017 16637-15846 263 DPV-W1170-84, 010 272 35 Soluble IFN-γ receptor B8R
COTV018 18372-16720 550 LSDV-NW-LW, 148 550 24 Kelch-like protein F3L
COTV019 20606-18897 569 YMTV-Amano, 007 637 26 Ankyrin repeat protein/PRANC domain B4R
COTV020 22308-21148 386 VACV-Cop, 254 353 36 Soluble IFN-α/β-receptor B19R
COTV021 22645-22839 64 — — — Hypothetical protein —
COTV022 22886-22716 56 VARV-BEN68-59, 008 58 76 Ankyrin repeat protein/orthopoxvirus C10L protein WR-018
COTV023 24921-22996 641 CPXV-BR, 027 632 62 Ankyrin repeat protein C9L
COTV024 25628-25185 147 VACV-WR, 021 150 56 Host range virulence factor C7L
COTV025 27243-26275 322 CPXV-FIN2000-MAN, 031 321 68 IL-1 receptor antagonist C10L/C4L
COTV026 28091-27303 262 CPXV-UK2000-K2984, 032 262 65 Complement binding protein C3L
COTV027 28833-28378 151 CPXV-GER1990-2, 036 176 70 Alpha-amanitin sensitivity protein N2L
COTV028 30193-29531 220 CPXV-NOR1994-MAN, 038 220 72 NF-κB inhibitor M2L
COTV029 30495-30247 82 CPXV-NOR1994-MAN, 041 88 64 IFN resistance/eIF2α-like PKR inhibitor K3L
COTV030 31457-30870 195 AMEV-Moyer, 173‡ 209 23 Hypothetical protein —
COTV031 32571-31462 369 VACV-GLV-1h68, 037 284 27 Ankyrin repeat protein/host range protein/NF-κB and PKR inhibitor K1L
COTV032 33750-32614 378 DPV-W848-83, 018 382 42 Serine protease inhibitor-like (SPI-3)/fusion regulatory protein K2L
COTV033 34038-33814 74 Anopheles darlingi, locus AND-04657/SPPV-A, 013 241/100 43/34 Epidermal growth factor-like domain C11R
COTV034 34694-34230 154 YMTV-Amano, 011 172 28 Apoptosis inhibitor F1L
COTV035 35163-34738 141 MYXV-Lau, 016 148 64 dUTPase F2L
COTV036 36780-35194 528 DPV-W848-83, 025 529 34 Kelch-like protein F3L
COTV037 37822-36827 331 DPV-W848-83, 026 321 74 Ribonucleotide reductase small subunit F4L
COTV038 38067-37825 80 SPPV-TU, 018‡ 79 34 Hypothetical protein —
COTV039 38487-38296 63 YLDV-Davis, 024 50 68 Unknown F8L
COTV040 39200-38550 216 DPV-W848-83, 030 215 56 S-S bond formation pathway protein F9L
COTV041 40515-39178 445 SWPV-Neb, 022 440 76 Serine/threonine kinase F10L
COTV042 41592-40516 358 DPV-W1170-84, 032 381 40 RhoA inhibitor/cell motility F11L
COTV043 43510-41579 643 DPV-W1170-84, 033 651 45 WV-associated protein F12L
COTV044 44654-43545 369 DPV-W848-83, 034 375 69 Palmitylated EV envelope protein F13L
COTV045 44835-44686 49 — — — Hypothetical protein —
COTV046 45278-44838 146 DPV-W848-83, 036 148 56 Unknown F15L
COTV047 46000-45350 216 SWPV-Neb, 028 217 36 Unknown F16L
COTV048 46060-46359 99 YMTV-Amano, 025 104 74 Nucleic acid binding phosphoprotein F17R
COTV049 47865-46453 470 SPPV-TU, 029 474 74 Poly(A) polymerase large subunit E1L
COTV050 50042-47862 726 SWPV-Neb, 031 732 54 WV assembly/interaction with F12 E2L
COTV051 50659-50081 192 SPPV-TU, 031 177 43 IFN resistance/dsRNA-binding protein/PKR inhibitor E3L
COTV052 51414-50776 212 RFV-Kas, 035 222 60 RNA polymerase 30-kDa subunit E4L
COTV053 51458-53161 567 DPV-W848-83, 044 566 70 Core protein/virus morphogenesis E6R
COTV054 53171-53977 268 DPV-W848-83, 045 267 80 Unknown E8R
COTV055 56994-53974 1006 LSDV-LW1959, 041 1006 70 DNA polymerase E9L
COTV056 57041-57328 95 RFV-Kas, 040 96 76 Sulfhydryl oxidase E10R
COTV057 59394-57337 685 DPV-W1170-84, 048 678 41 Unknown O1L
COTV058 59521-59417 34 MYXV-Lau, 042 32 68 MV entry-fusion complex O3L
COTV059 60481-59537 314 DPV-W848-83, 049 313 69 DNA-binding protein I1L
COTV060 60715-60482 77 SWPV-Neb, 040 75 56 Virus assembly, crescent formation I2L
COTV061 61532-60708 274 DPV-W848-83, 051 272 60 DNA-binding phosphoprotein I3L
COTV062 61830-61588 80 DPV-W848-83, 053 78 39 MV membrane protein I5L
COTV063 62986-61838 382 DPV-W848-83, 054 389 59 Telomere binding protein I6L
COTV064 64277-62979 432 DPV-W848-83, 055 432 76 Virion core protease I7L
COTV065 64287-66326 679 LSDV-LW1959, 051 683 67 RNA helicase/NPH-II I8R
COTV066 68128-66323 601 SWPV-Neb, 047 593 63 Metalloprotease G1L
COTV067 68460-68125 111 MYXV-Lau, 051 111 51 MV entry-fusion complex G3L
COTV068 68454-69128 224 SPPV-TU, 049 222 52 Viral late transcription elongation factor G2R
COTV069 69475-69092 127 SPPV-TU, 050 126 78 Glutaredoxin protein G4L
COTV070 69478-70788 436 DPV-W1170-84, 061 434 55 Flap endonucleases (FEN-1) G5R
COTV071 70791-70982 63 LSDV-Nee, 056 63 79 RNA polymerase subunit RPO7 G5.5R
COTV072 70982-71503 173 RFV-Kas, 056 174 63 NlpC/P60 superfamily G6R
COTV073 72597-71500 365 DPV-W848-83, 064 375 60 Assembly/seven-protein complex G7L
COTV074 72627-73406 259 LSDV-Nee, 059 260 81 Viral late transcription factor VLTF-1 G8R
COTV075 73416-74420 334 DPV-W1170-84, 066 335 61 MV entry-fusion complex G9R
COTV076 74421-75152 243 DPV-W1170-84, 067 249 79 Myristylated MV membrane protein L1R
COTV077 75164-75439 91 DPV-W848-83, 068 95 46 Virus assembly, crescent formation L2R
COTV078 76390-75422 322 LSDV-Nee, 063 318 72 Core protein, early transcription L3L
COTV079 76415-77176 253 DPV-W848-83, 070 252 80 DNA-binding virion protein VP8 L4R
COTV080 77195-77581 128 SPPV-TU, 061 132 56 MV entry-fusion complex L5R
COTV081 77538-77984 148 LSDV-LW1959, 067 148 72 Assembly/seven-protein complex J1R
COTV082 78003-78539 178 SWPV-Neb, 063 180 68 Thymidine kinase J2R
COTV083 78596-79108 170 YLDV-Davis, 069 178 43 Host range virulence factor C7L
COTV084 79181-80182 333 DPV-W1170-84, 075 374 76 Poly(A) polymerase small subunit J3R
COTV085 80097-80654 185 SWPV-Neb, 066 185 75 RNA polymerase subunit RPO22 J4R
COTV086 81064-80651 137 SWPV-Neb, 067 134 59 MV entry-fusion complex J5L
COTV087 81158-85024 1288 DPV-W1170-84, 078 1286 82 RNA polymerase subunit RPO147 J6R
COTV088 85536-85021 171 DPV-W1170-84, 079 172 80 Serine/tyrosine dual phosphatase H1L
COTV089 85551-86123 190 DPV-W1170-84, 080 190 67 MV entry-fusion complex H2R
COTV090 87106-86120 328 SWPV-Neb, 071 324 56 MV heparin binding surface protein H3L
COTV091 89500-87107 797 SWPV-Neb, 072 801 75 RNA polymerase-associated protein RAP94 H4L
COTV092 89869-90492 207 SWPV-Neb, 073 181 41 Viral late transcription factor VLTF-4 H5R
COTV093 90516-91460 314 SWPV-Neb, 074 320 68 DNA topoisomerase type I H6R
COTV094 91507-91962 151 SWPV-Neb, 075 149 55 Virus assembly, crescent formation H7R
COTV095 91963-94527 854 DPV-W848-83, 086 843 70 Capping enzyme large subunit D1R
COTV096 95079-94489 196 SWPV-Neb, 077 146 45 Assembly/seven-protein complex D2L
COTV097 94925-95677 250 SWPV-Neb, 078 244 41 Assembly/seven-protein complex D3R
COTV098 95674-96330 218 DPV-W848-83, 089 218 78 Uracil DNA glycosylase D4R
COTV099 96372-98735 787 DPV-W1170-84, 090 786 69 DNA-independent NTPase D5R
COTV100 98755-100647 630 LSDV-Nee, 085 635 86 Viral early transcription factor (VETF) small subunit D6R
COTV101 100650-101135 161 SWPV-Neb, 082 161 81 RNA polymerase subunit RPO18 D7R
COTV102 102071-101097 324 MYXV-Lau, 088 286 31 Carbonic anhydrase-like protein D8L
COTV103 102152-102784 210 DPV-W848-83, 093 211 59 Decapping enzyme/Nudix hydrolase motif D9R
COTV104 102781-103518 245 TANV-KEN, 091 255 58 Decapping enzyme/Nudix hydrolase motif D10R
COTV105 105424-103520 634 DPV-W1170-84, 095 635 72 NPH-I; transcription elongation, termination, release factor D11L
COTV106 106314-105460 284 SWPV-Neb, 086 287 73 Capping enzyme, small subunit D12L
COTV107 107980-106331 549 DPV-W848-83, 097 550 80 Crescent/IV scaffold protein; rifampin resistance D13L
COTV108 108460-108008 150 DPV-W848-83, 098 151 64 Viral late transcription factor VLTF-2 A1L
COTV109 109173-108499 224 MYXV-Lau, 095 224 87 Viral late transcription factor VLTF-3 A2L
COTV110 109397-109170 75 MYXV-Lau, 096 75 68 Thioredoxin-like protein A2.5L
COTV111 111374-109419 651 SWPV-Neb, 091 652 76 Core wall protein A3L
COTV112 111885-111424 153 DPV-W1170-84, 102 151 42 Core wall protein A4L
COTV113 111925-112428 167 YMTV-Amano, 089 165 57 RNA polymerase subunit RPO19 A5R
COTV114 113546-112425 373 DPV-W848-83, 104 374 74 Virion morphogenesis A6L
COTV115 115720-113570 716 DPV-W1170-84, 105 715 78 Viral early transcription factor (VETF) large subunit A7L
COTV116 115777-116555 292 MYXV-Lau, 102 286 66 Viral intermediate transcription factor VITF-3 A8R
COTV117 116896-116660 78 DPV-W1170-84, 107 81 76 MV membrane protein A9L
COTV118 119593-116897 898 DPV-W1170-84, 108 915 64 Core wall protein A10L
COTV119 119608-120537 309 RFV-Kas, 105 314 69 Virus assembly, crescent formation A11R
COTV120 121013-120546 155 YMTV-Amano, 096 167 61 Core structural protein A12L
COTV121 121243-121031 70 LSDV-Nee, 105 67 47 MV maturation protein A13L
COTV122 121579-121286 97 MYXV-Lau, 108 96 52 MV membrane protein A14L
COTV123 121757-121596 53 LSDV-Nee, 107 53 79 MV membrane protein, virulence factor A14.5L
COTV124 122028-121747 93 DPV-W848-83, 114 94 57 Assembly/seven-protein complex A15L
COTV125 123142-122012 376 DPV-W848-83, 115 380 60 MV entry-fusion complex A16L
COTV126 123789-123157 210 DPV-W848-83, 116 197 57 MV membrane protein A17L
COTV127 123804-125240 478 DPV-W1170-84, 117 482 60 DNA helicase, transcription elongation factor A18R
COTV128 125451-125221 76 DPV-W848-83, 118 75 66 Unknown A19L
COTV129 125795-125454 113 DPV-W848-83, 119 115 61 MV entry-fusion complex A21L
COTV130 125794-127089 431 DPV-W1170-84, 120 428 51 DNA polymerase processivity factor A20R
COTV131 127061-127564 167 DPV-W848-83, 121 181 79 Holliday junction resolvase A22R
COTV132 127587-128735 382 RFV-Kas, 118 385 59 Viral intermediate transcription factor VITF-3 A23R
COTV133 128762-132241 1159 DPV-W1170-84, 123 1155 83 RNA polymerase subunit RPO132 A24R
COTV134 132944-132231 237 DPV-W848-83, 124 137 45 MV attachment protein A27L
COTV135 133367-132945 140 DPV-W848-83, 125 140 69 MV entry-fusion complex A28L
COTV136 134290-133382 302 DPV-W848-83, 126 300 64 RNA polymerase subunit RPO35 A29L
COTV137 134486-134274 70 DPV-W848-83, 127 75 67 Assembly/seven-protein complex A30L
COTV138 134625-134494 43 LSDV-Nee, 122‡ 41 44 Hypothetical protein —
COTV139 134659-135417 252 DPV-W848-83, 129 254 82 DNA packaging protein A32L
COTV140 135513-136058 181 CMLV-M96, 154 184 33 EV envelope glycoprotein A33R
COTV141 136071-136610 179 SWPV-Neb, 121 169 37 EV envelope glycoprotein A34R
COTV142 136636-137196 186 SWPV-Neb, 122 185 42 MHC class II inhibitor A35R
COTV143 137204-138061 285 SWPV-Neb, 123 314 30 Concanavalin-like precursor —
COTV144 138116-138703 195 SWPV-Neb, 124‡ 199 26 WV transmembrane phosphoprotein A36R
COTV145 138735-139562 275 SWPV-Neb, 125 280 40 Unknown A37R
COTV146 140356-139559 265 MYXV-Lau, 133 299 29 CD47-like protein A38L
COTV147 140358-140765 135 DPV-W1170-84, 137 138 39 Unknown E7R
COTV148 140819-141226 135 SPPV-TU, 129 161 47 Superoxide dismutase-like protein A45R
COTV149 141254-142933 559 DPV-W848-83, 143 562 58 DNA ligase A50R
COTV150 142968-143522 184 DPV-W848-83, 146 188 37 Unknown —
COTV151 143628-144653 341 SPPV-TU, 134 335 36 Unknown A51R
COTV152 144592-145248 218 MYXV-Lau, 144 192 45 Toll/IL-1-receptor protein A52R
COTV153 145319-145783 154 LSDV-Nee, 138 186 32 Ig domain OX-2-like protein A56R
COTV154 145827-146585 252 YLDV-Davis, 145 309 62 Serine/threonine kinase B1R
COTV155 146697-147482 261 MYXV-Lau, 148 234 35 Ubiquitin ligase/host defense modulator —
COTV156 147526-147900 124 MYXV-SG33, 150 138 42 Apoptosis inhibitor/NF-κB inhibitor N1L
COTV157 147929-148804 291 SPPV-TU, 140 302 39 Tyrosine kinase-like protein —
COTV158 148846-150717 623 DPV-W848-83, 159 641 38 Ankyrin repeat protein/PRANC domain B4R
COTV159 150755-152152 465 LSDV-Nee, 150 447 25 Ankyrin repeat protein —
COTV160 152167-152511 114 DPV-W1170-84, 166 94 39 Unknown —
COTV161 152910-154166 418 CPXV-GER91, 042 424 51 Phospholipase-D-like protein/nicking-joining enzyme K4L
COTV162 154599-156752 717 MYXV-Lau, 152 675 21 Ankyrin repeat protein B4R
COTV163 157738-163266 1842 CPXV-AUS1999-867, 206 1924 57 Surface glycoprotein —
COTV164 163359-165197 612 LSDV-LW1959, 150 636 25 Ankyrin repeat protein/PRANC domain —
COTV165 165751-166758 335 CPXV-GER91-3, 195 346 55 Serine protease inhibitor (SPI-2/CrmA) B13R/B14R
COTV166 166831-167283 150 CPXV-GER91-3, 196 149 65 NF-κB inhibitor/virulence factor B15R
COTV167 167398-168453 351 CPXV-AUS1999-867, 204 373 57 Serine protease inhibitor-like SPI-1 C12L
COTV168 168481-169059 192 CPXV-BR, 014 202 42 TNF-like receptor protein (CrmB) C22L/B28R
COTV169 169738-170427 229 DPV-W1170-84, 009 244 45 Alpha-amanitin sensitivity protein N2L
COTV170 170429-171274 281 Leopardus pardalis, gene FLA-I/SQPV-I2L* 338/344 26/22 MHC class I antigen —
COTV171 171578-172432 284 ECTV-Mos, 004 273 29 Kelch-like protein A55R
COTV172 172718-173539 273 CPXV-UK2000-K2984, 009 273 31 Kelch-like protein A55R
COTV173 173862-174230 122 RFV-Kas, 004/163 125 49 Unknown —
COTV174 174284-174736 150 LSDV-LW1959, 001/161 159 47 NF-κB inhibitor/virulence factor B15R
COTV175 174777-175559 260 CPXV-GER91, 001/219 252 26 C-C chemokine binding protein (35-kDa major secreted virus protein) B29R
COTV176 175563-175895 110 Mus musculus, gene Scya2 148 25 C-C motif chemokine protein —
COTV177 175910-176941 343 MPXV-ZAR-1979, 005/187 354 37 Soluble IFNα/β receptor B19R
COTV178 176965-177339 124 YKV, 178 127 42 TNF receptor-like protein (CrmE) —
COTV179 177363-177866 167 CPXV-UK2000-K2984, 003 355 25 TNF receptor-like protein (CrmB) C22L/B28R
COTV180 177895-178527 210 Bos taurus, locus BOS_3916/CNPV-VR111, 170* 212/212 55/50 Thymidylate kinase A48R
COTV181 178580-180280 566 DPV-W848-83, 019 643 25 Ankyrin repeat protein/PRANC domain B4R
COTV182 180629-181423 264 CPXV-NOR1994-MAN, 002 245 28 Chemokine binding protein C23L/B29R
COTV183 181427-181729 100 Mustela putorius furo, gene CCL13 70 35 C-C motif chemokine protein —
COTV184 182276-183988 570 DPV-W1170-84, 019 643 25 Ankyrin repeat protein B4R
COTV185 184018-184569 183 CPXV-GER1980-EP4, 205 188 32 Serine protease inhibitor -like protein C13L/C14L
a

ORFs in boldface are conserved in all chordopoxviruses and encode proteins used for analysis of phylogeny.

b

Best-matching protein sequences obtained by BLASTP-NCBI. YKV, Yoka poxvirus; VARV, variola virus; ECTV, ectromelia virus; AMEV, Amsacta moorei entomopoxvirus; SPPV, sheeppox virus; TANV, tanapox virus; SQPV, squirrel poxvirus; CMLV, camelpox virus. Symbols: *, the highest score obtained with a cellular protein, but a significant match also with a poxvirus protein, which is indicated after a slash; ‡, no significant match was obtained using BLASTP-NCBI, and scores were obtained by searching the poxvirus database; —, no significant match.

c

% ID, percentage of amino acid identity.

d

PRANC, poxvirus protein repeats of ankyrin, C-terminal; TNF, tumor necrosis factor; Crm, cytokine response modifier; IL-1, interleukin-1; eIF2α, α subunit of eukaryotic initiation factor 2; dsRNA, double-stranded RNA; NPH, nucleoside triphosphate phosphohydrolase; NTPase, nucleoside triphosphatase.

e

—, no VACV-Cop ortholog. In the absence of an ortholog in VACV-Cop, an ortholog in VACV-WR is indicated if available.