Fig 10.
Structure of the complex formation between SIV Nefmac239 and CD3ζ. (A) Ribbon display of the core domain structure of SIV Nefmac239 (blue) with the first ITAM motif of CD3ζ indicated in orange (45). The hydrophobic residue Ile132 is located at the surface of Nef and is involved in tight interactions with the ζ chain of CD3. (B) Close-up view of CD3ζ-Nef binding. L75 and N76 of CD3ζ bind into a characteristic hydrophobic crevice formed by I132 of Nef. Similar interactions with receptor tail motifs are supposed for HIV-2 Nef. The mutation of I132T in HIV-2 Nef allele RH-2_A8 will disrupt the hydrophobic contacts formed between Nef and ζ, resulting in the impairment of CD3 downmodulation. Molecular diagrams were drawn using PyMOL (http://www.pymol.org/).