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. 2012 May;86(9):4921–4934. doi: 10.1128/JVI.06759-11

Fig 3.

Fig 3

NSP4 Cytotoxicity in E. coli is conserved across rotavirus groups. Tenfold serial dilutions of BL21(DE3) E. coli bearing expression plasmids for the indicated NSP4 were plated on LB-Amp supplemented with 1 mM IPTG and incubated overnight at 37°C. Error bars represent standard deviations. (A) Full-length RVA, RVB, and RVC NSP4s expressed in E. coli compared to the noncytotoxic RVA NSP4 with the aa 95–146 truncation. (B) SA11 and Ty-1 aa 20–146 truncation constructs and the Ty-1/SA11-PD chimera, expressed in E. coli. *, P < 0.05.