Fig 7.

Subcellular localization of NSP4 using organelle-specific markers. Markers for the endoplasmic reticulum [calnexin (A to D) and Sec61β (E to H)], ER-Golgi intermediate compartment [ERGIC-53 (I to L)], Golgi [giantin (M to P)], and autophagosomes [LC3 (Q to T)] were used to examine the subcellular localization of RFP-tagged SA11 (A, E, I, M, and Q), Ty-1 (B, F, J, N, and R), RVB (C, G, K, O, and S), or RVC NSP4 (D, H, L, P, and T). Calnexin, ERGIC-53, and giantin were detected by indirect immunofluorescence as described in Materials and Methods. Sec61β and LC3 were tagged with EGFP. For confocal microscopy, TO-PRO-3 was used to stain nuclei, which is pseudocolored blue. Bars, 10 μm.