Table 3.
Summary of NSP4 viroporin activitya
| Group | Construct | E. coli lysis | Cytotoxicity | Altered redox activity (RSG) | Membrane perm. (PI) | Localization | Elevation of [Ca]cyto |
|---|---|---|---|---|---|---|---|
| RVA (E2) | SA11 1-175 (FL) | No | Yes | + | + | LC3 puncta | Yes |
| SA11 20-146 | Yes | Yes | + | + | NA | NA | |
| RVA (murine) (E7) | EC 20-146 | Yes | Yes | + | + | NA | NA |
| RVA (avian) (E4) | Ty-1 20-146 | No | Yes | + | + | LC3 puncta | Yes |
| RVA chimera | Ty-1/SA11-PD 20-146 | Yes | Yes | ND | ND | NA | NA |
| RVB (rat) | IDIR 1-218 (FL) | No | Yes | + | − | LC3 puncta | Yes |
| RVC (porcine) | Cowden 1-150 (FL) | Yes | Yes | + | + | LC3 puncta | Yes |
a
The viroporin activities observed with each NSP4 in the different assays are summarized here. Boldface type indicates data not shown. FL, full-length constructs; ND, not done; NA, not applicable; RSG, Redox Sensor Green; perm, permeabilization; PI, propidium iodide.