Fig 10.

Digital confocal microscope images showing the localization of ICP4 and ICP8 in Vero cells infected with wild-type HSV-1(F), YK451 (ΔVP22), YK452 (ΔVP22-repair), YK455 (VP22LL251AA), YK456 (VP22LL251AA-repair), or YK476 (ΔUL41) at an MOI of 1. Infected Vero cells were fixed at the indicated times postinfection, permeabilized, stained with an antibody to ICP4 or ICP8, and examined by confocal microscopy. Left and right columns at each time point show protein fluorescence and simultaneous acquisition of protein fluorescence and digital interference contrast, respectively.