Fig 6.
Effects of the ΔVP22, VP22LL235AA, and VP22LL251AA mutations on the production of plaques. Vero cells were infected with wild-type HSV-1(F), YK451 (ΔVP22), YK452 (ΔVP22-repair), YK453 (VP22LL235AA), YK454 (VP22LL235AA-repair), YK455 (VP22LL251AA), or YK456 (VP22LL251AA-repair) at an MOI of 0.0001 under plaque assay conditions. The diameters of 20 single plaques for each of the recombinant viruses were determined 2 days after infection. The data shown are means and standard errors. Asterisks indicate significant differences (*, P < 1 × 10−8) by a two-tailed Student t test.