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Immunoblots of electrophoretically separated lysates of Vero cells that were either mock infected (lane 7) or infected with wild-type HSV-1(F) (lane 1), YK451 (ΔVP22) (lane 2), YK453 (VP22LL235AA) (lane 3), YK454 (VP22LL235-repair) (lane 4), YK455 (VP22LL251AA) (lane 5), or YK456 (VP22LL251AA-repair) (lane 6) at an MOI of 1. Infected Vero cells were harvested 20 h postinfection and were immunoblotted with an antibody to VP16, gB, VP22, ICP0, ICP4, ICP27, UL41, or α-tubulin.
