Table 1.

Primer sequences used for the construction of recombinant viruses

Mutation	Sequence
ΔVP22	5′-CCGTCCGAACCCAGGCCTAATTGTCCGCGCATCCGACCCTAGCGTGTT CGTGGAACCATGTAAAGGATGACGACGATAAGTAGGG-3′
	5′-TTCACGGAGCGGCGAGAGGTCATGGTTCCACGAACACGCTAGGGTCG GATGCGCGGACAACAACCAATTAACCAATTCTGATTAG-3′
VP22LL235AA	5′-GGACATGTCGCGTCCGCGCACAGACGAAGACCTCAACGAAGCCGCTG GCATCACCACCATCCGCGTAGGATGACGACGATAAGTAGGG-3′
	5′-TTTTGCCCTCGCAGACCGTCACGCGGATGGTGGTGATGCCAGCGG CTTCGTTGAGGTCTTCGTCTGCAACCAATTAACCAATTCTGATTAG-3′
VP22LL235AA-repair	5′-GGACATGTCGCGTCCGCGCACAGACGAAGACCTCAACGAACTCCTTG GCATCACCACCATCCGCGTAGGATGACGACGATAAGTAGGG-3′
	5′-TTTTGCCCTCGCAGACCGTCACGCGGATGGTGGTGATGCCAAGGAGT TCGTTGAGGTCTTCGTCTGCAACCAATTAACCAATTCTGATTAG-3′
VP22LL251AA	5′-CATCACCACCATCCGCGTGACGGTCTGCGAGGGCAAAAACGCGGCTC AGCGCGCCAACGAGTTGGTAGGATGACGACGATAAGTAGGG-3′
	5′-CCTGCACCACGTCTGGATTCACCAACTCGTTGGCGCGCTGAGCCGCG TTTTTGCCCTCGCAGACCGCAACCAATTAACCAATTCTGATTAG-3′
VP22LL251AA-repair	5′-CATCACCACCATCCGCGTGACGGTCTGCGAGGGCAAAAACCTGCTTC AGCGCGCCAACGAGTTGGTAGGATGACGACGATAAGTAGGG-3′
	5′-CCTGCACCACGTCTGGATTCACCAACTCGTTGGCGCGCTGAAGCAGGT TTTTGCCCTCGCAGACCGCAACCAATTAACCAATTCTGATTAG-3′
ΔUL41	5′-GACCGTCGCACCAACCACGCCTTAGTTAGGCCGATCCGCAGTTACAAT TGACCTGACATGTAGAGGATGACGACGATAAGTAGGG-3′
	5′-TTCATCATCCCGAACAAACCCATGTCAGGTCAATTGTAACTGCGGAT CGGCCTAACTAAGCAACCAATTAACCAATTCTGATTAG-3′