Fig 1.
(A) BIAcore sensorgram showing the equilibrium dissociation constant (KD) of UCLA1 from a synthetic HIV-1 ConC gp120. The aptamer was simultaneously injected over the immobilized gp120 at ½ log10 dilutions with the initial concentration at 500 nM followed by 100 nM, 20 nM, 4 nM, and 1 nM. (B) BIAcore sensorgram showing the KD of B40t77 aptamer from HIV-1 ConC gp120. The aptamer was simultaneously injected over the immobilized gp120 at 2-fold dilutions with the initial concentration at 2,000 nM, followed by 1000 nM, 500 nM, 125 nM, and 31 nM.