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. 2012 May;86(9):4833–4843. doi: 10.1128/JVI.05507-11

Fig 1.

Fig 1

Binding of recombinant RV VP8* to synthetic oligosaccharides. Recombinant proteins with the full-length or the core region of the RV VP8* from seven P[8] or P[4] human RVs were tested for binding to oligosaccharides with (A) or without (B) removal of the GST tag. Except for BM13851 VP8* core-GST to H type 1-PAA and BM13851 VP8* core-GST, BM151 VP8*-GST, and BM5265 VP8* core-GST to Leb-PAA, all VP8*- and VP8* core-GST proteins showed significant binding to Leb and H type 1 antigens compared with binding to Lex-PAA (means + 2 standard deviations [SD]). (C) One of each of the P[4] (DS1) and P[8] (RVP) RVs was tested with extended oligosaccharides representing variable HBGAs from two different sources (GlycoTech Corp. and the CFG) to confirm the binding specificities to the Leb and H type 1 antigens.