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Binding of RV-VP8* to milk Leb antigen in size-exclusive FPLC fractions. Boiled FPLC fractions of one secretor's milk sample (milk 36) were used to coat microtiter plates at a dilution of 1:15 in 1× PBS. The bound BM13851 VP8*-GST protein was detected by rotavirus hyperimmune sera. Only the high-molecular-weight fractions containing Leb antigen showed binding. The distribution of milk Leb in the FPLC fraction was previously measured in a separate experiment.
