Fig 1.

Matrix detachment upregulates PDK4 in mammary epithelial cells. (A) Quantitative RT-PCR measurement of individual PDKs in HMEC under attached (Att) and suspended (Susp) conditions. (B) Time course of PDK4 induction in HMEC under suspension. (C) Quantitative RT-PCR analysis of expression of PDKs in MCF10A cells. (D) Induction of PDK4 in suspended MCF10A cells at the indicated time points. (E) Quantitative RT-PCR analysis of absolute levels of PDKs in attached and suspended MCF10A cells. cDNA plasmids of each PDK were used as references. (F) Northern blotting of PDK4 in attached and suspended MCF10A cells. (G) Immunoblotting of PDKs, LDHA, and tubulin in attached and suspended MCF10A cells.