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. 2012 May;32(10):1928–1943. doi: 10.1128/MCB.06561-11

Fig 1.

Fig 1

Skp2 regulates ERα protein levels and stability in breast cancer cells. (A) Immunohistochemical staining for Skp2 and ERα proteins in ER-negative and -positive human breast tumors. (B) Western analysis of breast cancer cell lines. (C) Microarray data analysis of 209 ERα-positive and 77 ERα-negative human breast tumors (56) showing the relationship of Skp2 with ERα, tumor grade, and survival. (D) Western analysis of MCF-7 cells transfected with the control or Skp2 siRNA, followed by ligand treatment. Veh, vehicle. (E) Western analysis of MCF-7 cells infected with the control, Skp2-WT, or Skp2-ΔFbox adenovirus with or without MG-132. (F) Western analysis of MCF-7 cells infected with the control or Skp2 adenovirus and treated with cycloheximide (50 μg/ml) for the indicated times.