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Replacing either the first, second, or both MP2s with M5. (A) Four combinations of MP2 and M5 inserted in the DT40 Ig lambda locus. (B) Transcription levels in the IgL V region in the four knock-in clones. The histograms show mRNA levels relative to those of the MP2-MP2 knock-in clone; the values are normalized to chicken β-actin levels. The data represent means and standard deviations (SD) of three independent experiments.
