Fig 3.

RBP4 activates proinflammatory signaling pathways in macrophages. (A) Western blot of total and phosphorylated IKKα/IKKβ from RAW264.7 macrophages treated with holo-RBP4 (50 μg/ml) for the indicated times. (B) Quantification of NF-κB promoter activity in RAW264.7 macrophages transfected with pNFκB-Luc reporter plasmid and then treated with holo-RBP4 (50 μg/ml for 18 h). LPS (10 ng/ml) was used as a positive control. AU, arbitrary units. Data are expressed as the mean ± SE and normalized to β-galactosidase (β-Gal) expression. *, P < 0.05 versus PBS. (C) Western blot of total and phosphorylated Akt, ERK, p38, and JNK from RAW264.7 cells that were treated with either apo- or holo-RBP4 (50 μg/ml) for the indicated times.