Fig 8.

Overexpression of Mcl-1 in HCT116 prevents CIS in xenograft tumor-bearing nude mice. (A) Growth curves of HCT116 vector control or Mcl-1 overexpressing tumors in nude mice. A total of 1 × 10⁷ cells were injected into the right dorsal flank of female mice, and after 10 days, the animals were distributed into groups receiving PBS alone (no treatment) or 1.2 mg/kg of doxorubicin (Dox) in PBS every third day intraperitoneally (n = 5). Data points are mean ± SD values of tumor volumes. *, P value of <0.05 between doxorubicin-treated and untreated groups. (B) Immunohistology of HCT116 vector and Mcl-1up tumors. SA-β-gal, Ki-67, and TUNEL staining were done using formalin-fixed and frozen sections. (C) The percentage of Ki67⁺ cells was determined in the indicated tumor sections. Data are presented as mean ± SD. **, P value of <0.001 between the doxorubicin-treated and untreated vector control tumors. Data are representative of three independent studies.