Fig 2.

Nrd1 regulates cell size. (A and B) nrd1-102HA cells are larger than NRD1HA cells. Regions of identical sizes are shown. (C) The number of pixels making up the area of each of 100 cell images from each strain is plotted in order of size. (D) PAR-CLIP data showing that Nrd1 and Nab3 bind to the 5′ end of the XBP1 transcript. Vertical lines indicate the numbers of transcripts bound to the sense strand RNA at each location by Nrd1 and Nab3 per 10⁷ aligned PAR-CLIP sequencing reads (19). (E) XBP1 is overexpressed in nrd1-102HA cells. Total RNA was analyzed by quantitative RT-PCR using primer sequences near the 3′ end of XBP1.