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Ras overactivation causes a synthetic slow-growth phenotype in nrd1-102 cells. (A) Schematic representation of the Ras pathway. Genes identified in the dSLAM screen are outlined in gray. (B and C) Confirmation of the synthetic slow-growth phenotype of dSLAM hits. (D) Overexpression of PDE2 partially rescues nrd1-102HA. (E) Synthetic slow growth of bcy1Δ nrd1-102 cells For panels B to E, strains were serially diluted at equal densities, spotted onto plates, and grown as indicated.
