Fig 6.

Nrd1-Nab3 rapidly represses TYE7 and DLD3 in response to nutrient depletion. (A and B) PAR-CLIP data showing Nrd1 and Nab3 binding to the 5′ end of TYE7 (A) and DLD3 (B) transcripts. Vertical lines indicate the numbers of transcripts bound at each location by Nrd1 per 10⁷ aligned sequencing reads (19). (C to E) TYE7 is more overabundant in nab3-42 cells after transfer from YPD at 28°C to water at 37°C (C) or from SC with glucose at 28°C to SC lacking glucose at 37°C (E) than after the temperature change alone (D). (F and G) DLD3 is more overabundant in nab3-42 cells after transfer from YPD at 28°C to water at 37°C (F) than after the temperature change alone (G). (H) TYE7 is also overabundant in nrd1-102 relative to NRD1 cells after transfer from YPD at 28°C to water at 37°C. In panels C to H, the numbers above each pair of columns represent fold overabundances of the target mRNA in the temperature-sensitive mutant compared to the wild type at each time point. Total RNA was collected before the medium transfer and at 20-min intervals and analyzed by qRT-PCR. Relative expression was normalized to the expression level of the 18S rRNA gene and was set to 1 for the initial wild-type sample. Error bars represent standard errors of the means from two independent experiments.