Fig 2.

Impaired proliferation and block in G₁-S transition in ADAP^−/− T cells upon antigen challenge in vivo. (A) Naïve DO11.10 hCAR control (Ctrl) or DO11.10 hCAR/ADAP^−/− T cells were transduced with control adenovirus expressing Thy1.1 (Thy) or adenovirus expressing Thy1.1 and wild-type ADAP (WT), ADAPΔCAR mutant (ΔCAR), ADAPΔTAK mutant (ΔTAK), or ADAPΔSKAP mutant (ΔSKAP), stained with CFSE, and then transferred into BALB/c mice. Two hours after T cell transfer, host mice were injected with OVAp and spleens were harvested 42 h after OVAp injection. T cells expressing Thy1.1 and the DO11.10 T cell receptor were identified by flow cytometry and analyzed for CFSE dye dilution. (B) CD4 T cells transduced and transferred as described for panel A were analyzed for cell cycle transition using DAPI staining. Results for both the proliferation and cell cycle analysis are representative of at least three independent experiments.