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Differential binding of HCR/A and HCR/T to SV2. FLAG-HCR/A or FLAG-HCR/T (5 μg) was incubated with 10 μg of an SV cell lysate. Bound HCR was extracted with anti-FLAG agarose beads. HCRs were eluted with FLAG peptide, and eluted material was analyzed by Western blotting. Quantitation showed that in the CHAPS cell lysate SV2B bound to HCR/T was ∼2% of SV2B bound to HCR/A.
