Fig 8.

TeNT cleavage of VAMP2 in rat cortical neurons. Rat cortical neurons were incubated alone (mock) or with 20 nM TeNT in high K buffer (High K), low K buffer (Low K), or high K/EGTA buffer (EGTA) for 20 min at 37°C. Cells were washed and incubated in conditioned neurobasal medium for an additional 18 h. Cells were fixed and processed for VAMP2 content by IF. VAMP2 cleavage was assayed with mouse anti-VAMP2 antibody (1:500 dilution; SYSY clone 69.1), which recognizes only intact VAMP2. Data are representative of 5 random fields from two independent experiments. *, P < 0.05; ***, P < 0.001. Error bars indicate standard deviations.