Fig 3.
Expression of litR mRNA in V. salmonicida LFI1238 as determined by qRT-PCR. The wild type was cultivated in LB medium supplemented with 1% NaCl and 3% NaCl and harvested at different optical densities (at 600 nm). The relative expression (RQ) was calculated by the 2−ΔΔCT method to determined the level of expression relative to the transcription level in cultures harvested at an OD600 of 0.1 in LB1 and LB3, respectively (38). The CT values were normalized using the 16S rRNA level of V. salmonicida as a reference.