Fig 2.

Intracellular colonization of FC-PD07i cell layers. PD07i cell layers were infected with UTI89-pEGFP in FCs as described in the text, with subsequent gentamicin treatment followed by inspection with CLSM (A and B) or fluorescence phase-contrast microscopy (C). For the CLSM images, cytoskeleton and membrane boundaries were visualized by Acti-stain 555 and cell nuclei were visualized using the background laser signal (red). (A) Invaded binuclear PD07i cell with cytosolic UTI89-pEGFP. Bacteria were coccoid in shape, as shown in the inset. Vertical and horizontal cross-sections of the Z-stack are shown. (B) Invading UTI89-pEGFP appears to be localized to vesicle compartments. The image at the right shows the actin stain alone, indicating vesicle boundaries (arrows). (C) late-stage intracellularly colonized PD07i cell. Bars, 10 μm.