Table 1.
Oligonucleotide sequences used for genotyping of TLR2a
| Target | Primer pair | Direction | Sequence |
|---|---|---|---|
| TLR2 gene | |||
| 2029C>T (677Arg→Trp) | P1 | Forward | 5′-ATTTGTTTCTTACAGTGAGCGGG-3′ |
| Reverse | 5′-GGAAATGGGAGAAGTCCAGTTC-3′ | ||
| P2 | Forward | 5′-CCTCCCTCTTACCCATGTTACTA-3′ | |
| Reverse | 5′-TGCACCACTCACTCTTCACA-3′ | ||
| 2258G>A (753Arg→Gln) | P3 | Forward | 5′-TTCAAGTTGTGTCTTCATAAGCG-3′ |
| Reverse | 5′-CAGATTTACCCAAAATCCTTCC-3′ | ||
| TLR2 pseudogene (exon 3 noncoding duplication) | P4 | Forward | 5′-TTCCCCTTTGAGAAGAAGCA-3′ |
| Reverse | 5′-GAGAGTTCCCTCACCCCTTC-3′ |
a
The genotyping method used for the 1892C>A (631 Pro→His) TLR2 polymorphism was a TaqMan C_25607736_10 SNP assay.