Fig 1.

Relative expression of ompU in wild-type and ΔtoxRS strains. Wild-type and ΔtoxRS cells were grown for 4 h in either LB, 3% NaCl, pH 7 or LB, 3% NaCl, pH 7 plus 30 min in LB, 3% NaCl, pH 5. Bars represent the relative expression of ompU normalized to 16S rRNA and relative to expression levels found in wild-type V. parahaemolyticus grown for 4 h in LB, 3% NaCl, pH 7. RNA was extracted for each condition on two separate occasions, and qPCR was performed in duplicate for each sample. Error bars indicate standard deviations. An unpaired Student's t test was used to compare differences in ompU expression under acid conditions and in the ΔtoxRS strain to expression in wild-type cells grown in LB, 3% NaCl, pH 7. *, P < 0.05; ***, P < 0.001.