A cartoon representation of the model containing two metal binding sites. The scheme has 5 thermodynamic “species”, P, Q, R, S and free metal, Cd. The scheme has 5 NMR species α, β, γ, δ and ε. The direct communication between species Q and R (“Cd internalization”) is important for the kinetics of metal binding but is thermodynamically irrelevant. In the cartoons, cadmium(II) is represented as a green circle, Cys-SH groups as yellow circles, Glu-COO− groups as red circles and Lys NH3+ groups as blue circles.