Figure 1.
Schematic of recording arrangements. A, The STNS during normal ongoing pyloric activity. Intracellular axon recordings were obtained from the dvn, 0.5–2 cm distal to the STG. PD axons were identified by their characteristic waveform and correspondence of spike patterns with a distal extracellular recording from the pdn. Only one of the two bilaterally projecting PD neurons is shown. CoG, Commissural ganglion; OG, esophageal ganglion. B, Antidromic stimulation and quantification of spike waveform parameters. For patterned stimulation and quantification of delay and waveform parameters, centrally generated rhythmic activity was blocked by applying 1 μm TTX to a petroleum jelly well built around the STG. Spikes were then elicited by electrically stimulating the pdn through the extracellular electrodes. The example trace indicates the measurements taken. Peaks were detected as voltage maxima in the intracellular recording, and the delay from stimulation to peak was measured. Troughs were detected as voltage minima between spikes or immediately before a spike. Voltage values of both peaks and troughs, as well as the spike duration (at of the amplitude) were measured.