Table 1.
Specific ADO A1, A2A, A2B, and A3 receptor primers for RT-PCR
| Receptor | Sequence of primer | bp | Size | Accession number |
|---|---|---|---|---|
| A1 | ||||
| Forward | 5′-GCCACAGACCTACTTCCA-3′ | 462–479 | 314 | XM_001687 |
| Reverse | 5′-TCCTGGCTGCCTTTATCTA-3′ | 758–775 | ||
| A2A | ||||
| Forward | 5′-AACAGCAACCTGCAGAACGTCA-3′ | 380–401 | 399 | XM_000675 |
| Reverse | 5′-TCCTGGCTGCCTTTATCTA-3′ | 755–778 | ||
| A2B | ||||
| Forward | 5′-CAAGTCACTGGCTATGATTGT-3′ | 173 | Olanrewaju et al. (50) | |
| Reverse | 5′-ATAGACAATGGGATTGACAAC-3′ | |||
| A3 | ||||
| Forward | 5′-TTCCGTCATGAGGATGGA-3′ | 12–19 | 236 | AY 011241 |
| Reverse | 5′-TCCTGGCTGCCTTTATCT A-3′ | 229–247 |