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. 2001 May 1;98(11):6372–6377. doi: 10.1073/pnas.091113598

Figure 2.

Figure 2

The antiproliferative effect of dizocilpine (●) and GYKI52466 (○) is attributed to decreased cell division and to increased cell death. (A) Human lung carcinoma (A549) cells were grown in culture medium only (control), in the presence of dizocilpine (100–500 μM), or in the presence of GYKI52466 (100–500 μM) for 96 h. BrdUrd incorporation was used as a marker for cell division. Cell death was quantified by measuring LDH release from damaged cells after 96 h in culture. The data represent mean normalized optical densities ± SEM of 6–8 trials. *, P < 0.05; **, P < 0.01; ***, P < 0.001 vs. control, Student's t test. (B) The effect of exposure of human lung carcinoma (A549) cells to different concentrations of dizocilpine or GYKI52466 on the numbers of trypan blue-accumulating cells is presented. Bars represent mean ± SEM number of trypan blue-positive cells/100 counted cells after 96 h in culture in the absence (control) or in the presence of different concentrations (μM) of glutamate antagonists (n = 6). **, P < 0.01; ***, P < 0.001, Student's t test.