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. 2010 Jul;2(2):102–106.

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Copyright © 2010 Park-media Ltd.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4 — (A) RT-PCR assay of gene transcripts, which are the markers of human embryonic stem cells, in embryonic fibroblasts (MA N1), human embryonic stem cells (HUES9), and four iPSC lines: hiPS-A24, hiPS-A29, hiPS-21L, and hiPS-30L; (B) embryoid bodies formed following the passage of hiPS-30l iPSC cells into a liquid medium, scale 100 µm; (C) RT-PCR assay of gene transcripts, which are the markers of three germ layers (ectoderm, mesoderm, and endoderm), following the differentiation of the hiPS-30L clone via the embryoid body formation.