Table 2.
Group of mice | Number of mice | First immunization | Second immunization | Third immunization | Influenza virus challenge | ||
---|---|---|---|---|---|---|---|
A/Duck/Potsdam/1402-6/1986 (Н5N2) | A/California/04/2009 (H1N1) | A/PR/8/34 | |||||
Experimental (М2sНВс) | 60 | 60 mice with TiterMax Gold Adjuvant 50 μg/mice s.c. (1) | 60 mice with Freund's incomplete adjuvant 50 μg/mice s.c. | 60 mice with Freund's incomplete adjuvant 50 μg/mice s.c. | 20 mice 5 LD/50 | 20 mice 5 LD/50 | 10 mice 5 LD/50 |
Control | 40 | PBS | PBS | PBS | 15 mice 5 LD/50 | 15 mice 5 LD/50 | 10 mice 5 LD/50 |
subcutaneous injection