Fig. 1.

a and b – Heteronuclear NMR spectrum ¹ H- ¹⁵ N HSQC of ¹⁵ N-labeled GpAtm in DPC micelles and DMPC/DHPC (1/4) bicelles, respectively, with a molar peptide/protein ratio of 1:35, at 40°C and pH 5.0. The resonance assignments are shown. c – The intermolecular proton-proton NOE contacts are presented on 2D project of the 3D ¹⁵ N, ¹³ C F1-filtered/F3-edited-NOESY spectrum acquired for the “isotopic-heterodimer” GpAtm sample embedded into the DPC micelles. The NOE cross peaks from the side chain hydroxyl ОγН group of T ⁸⁷ are labeled by arrows, revealing the intramolecular hydrogen-bonding of the hydroxyl group with the carbonyl group of G ⁸³ in major conformation of the GpAtm dimer.