Table 1.
Parameters | DNA samples | DNA quantity taken in reaction (pg) | Mean | Standard deviation | Coefficient of variation | |||
500 | 400 | 300 | 200 | |||||
∆C’T | C. kolensis before CD | -5.08 | -4.99 | -4.93 | -4.88 | -4.97 | ±0.09 | 1.7% |
C. insignis | -2.96 | -2.72 | -2.67 | -2.63 | -2.75 | ±0.15 | 5.4% | |
2–∆C’T | C. kolensis before CD | 33.82 | 31.78 | 30.48 | 29.45 | 31.38 | ±1.88 | 6.0% |
C. insignis | 7.78 | 6.59 | 6.36 | 6.19 | 6.73 | ±0.72 | 10.7% | |
Relative number of copies (post diminutedC. kolensisserves as a reference sample) | C. kolensis before CD | 528.01 | 496.16 | 475.86 | 459.78 | 489.95 | ±29.41 | 6.0% |
C. insignis | 121.48 | 102.86 | 99.36 | 96.64 | 105.09 | ±11.22 | 10.7% |