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. 2010 Oct-Dec;2(4):52–57.

Table 1.

Relative quantification of rRNA genes copy number amounts in prediminuted genomes of C. kolensis and soma of C. insignis using the 2 –∆C’T method

 Parameters DNA samples DNA quantity taken in reaction (pg) Mean Standard deviation Coefficient of variation
500 400 300 200
∆C’T C. kolensis before CD -5.08 -4.99 -4.93 -4.88 -4.97 ±0.09 1.7%
C. insignis -2.96 -2.72 -2.67 -2.63 -2.75 ±0.15 5.4%
2–∆C’T C. kolensis before CD 33.82 31.78 30.48 29.45 31.38 ±1.88 6.0%
C. insignis 7.78 6.59 6.36 6.19 6.73 ±0.72 10.7%
Relative number of copies (post diminutedC. kolensisserves as a reference sample) C. kolensis before CD 528.01 496.16 475.86 459.78 489.95 ±29.41 6.0%
C. insignis 121.48 102.86 99.36 96.64 105.09 ±11.22 10.7%