Fig. 1.

Rabin8 overexpression suppresses mTORC1 activity in HEK293 cells ( lanes 4, 6 ). The cells were transfected with control vector pcDNA3.1 ( lanes 1, 3, 5 ) or with Rabin8 ( lanes 2, 4, 6 ). Activity of mTORC1 was analyzed by levels of ribosomal protein S6 phosphorylation with phospho-specific antibody against S6 (Ser235/Ser236) in the presence of growth factors ( lanes 3,4 ), absence of growth factors ( lanes 3, 4 ), and after 15-min stimulation of cells grown in the growth-factor-free medium with the medium containing growth factors ( lanes 5,6 ). The levels of Rabin8 and β-аctin were measured using immunoblot analysis with specific antibodies.