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. 2011 Jul-Sep;3(3):77–84.

Fig. 2.

Fig. 2

Efficiency of production of TRX-tmFGFR3 fusion proteins in M9 minimal salt and М5052, С750501’ and M50501 auto-induction media. Coomassie blue-stained 14% Tris-glycine SDS-PAGE shows the fractionation of the lysate of whole cells producing TRX-tmFGFR3-nat, TRX-tmFGFR3-E and TRX-tmFGFR3-R. Recombinant strains were grown in: 1 – M9 medium, 13°C after induction with 0.05 mM IPTG; 2 – M5052 auto-induction medium, 18°C; 3 – M50501 auto-induction medium, 18°C; and 4 – С750501’ auto-induction medium, 18°C. Equivalents of 20 μL of cell culture were loaded into each lane. Protein molecular weight markers: 116.0, 66.2, 45.0, 35.0, 25.0, 18.4, and 14.4 kDa (top-down).