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. 2011 Jul-Sep;3(3):77–84.

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Copyright © 2011 Park-media Ltd.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3 — Efficiency of production of TRX-tmFGFR3-E target fusion proteins in rich (TB) and minimal (M9) media depending on growth temperature (37°C, 25°C, 13°C) after IPTG induction. Coomassie blue-stained 14% Tris-glycine SDS-PAGE analysis of TRX-tmFGFR3-E cell lysate (0.05 mM IPTG). Protein molecular weight markers (kDa) are shown on the right. The arrow on the left indicates TRX-tmFGFR3-E target fusion protein. 5 (TB) or 10 μL (M9) of cell culture were loaded into each lane. T — total cellular protein; S — soluble protein fraction.