Table 2.

Nucleotide sequences of the variable regions of 10 monoclonal neutralizing antibodies that had the highest avidity for pdmH1N1 hemagglutinin and that were studied in detail.

V2-36 pHC-2-36H: ATGAAACATCTGTGGTTCTTCCTTCTGCTGGTGGCGGCTCCCAGATGGGTCCTGTCCGAGCTGCGGCTGCACGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGGGACCCTGTCCCTCACGTGCACTGTGTCTGGTGGCTCCATCAGCGGAGGTAGTCATTACTGGGCCTGGATCCGCCAGTCCCCAGGGAAGGGCCTGGAGTGGATTGGAAGTATCTACTATAGTGGAAGCACCTACGACAGCCCGTCCCTCAAGAGTCGACTCAGCATGTCCGTGGACAAGTCGAAGAACCAGTTCCACCTGACGCTGAGGTCTGTGACCGCCGCAGATACGGCTGTTTATTTTTGTGCGAAACACGAATCTGATAGTAGCAGTTGGCACACTGGGTGGAACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG

pLcL-2-36L: ATGGCCTGGACTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGTCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCTACTCCAACATCGGGACAGGTTTTGATGTACACTGGTACCAGCATCTTCCAGGCAAAGCCCCCAAGCTCCTCATCTTTGGTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGCCTCCAGCCTGAGGATGAGGGTGACTATTACTGTCAGTCCTTTGACAGTAGCCTGAGTGGTTCGAACGTCTTCGGGACTGGGACCAAGCTGACCGTCCTAA

V2-7 pHC-2-7H: ATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTCCTGTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGACTTCGGAGACCCTGTCGCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAGGTGGGACTAACTACTGGGCCTGGATCCGCCAGCCCCCAGGGAAGGGGCCGGAGTGGCTTGGGAGTGTCTATTACAGTGGGAGCACCTACGACAACCCGTCCCTCAAGAGTCGAGTCAGCATATACGTAGACACGTCCAAGAACAAGTTCTCCCTGAGGCTGCGCTCTGTGACCGCCGCAGACACGGCTATTTATTACTGTGCGAGACATGAATCTGATAGTAGTAGTTGGCACACTGGGTGGAACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG

pLcL-2-7L: ATGCCCTGGGCTCTGCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTATGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGACAGAGGGTCACCATCTCCTGCACTGGGAGCAGTACCAACATCGGGGCAGGTCTTGCTGTCCACTGGTACCAGCATCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGCTTCTCTGGCTCCAAGTCTGGCACCACAGCCTCCCTGGCCATCACTGGGCTTCAGGCTGACGATGAGGCTGATTATTACTGCCAGTCCTTTGACGGCAGCCTGAGTGGTTCGAACGTCTTCGGAACTGGGACCAAGGTGACCGTCCTCA

V3-2G6 pHC-3-2G6H: ATGGACTGGACCTGGAGCGTCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTGCAGCTGGAGCAGTCTGGGGCTGAGGTGAAGAGGCCTGGGTCCTCGGTGAAGGTCTCCTGCCAGACTTCTGGAGGCACCTTCAGCAGTTTTGCTTTCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGAGGGATCATCGGTATGTTTGGGACAACAAGCTACGCACAGAAGTTCCAGGGCAGAGTCACGATTTCCGCGGACGAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGACGACACGGCCATATATTACTGTGCGAGAGGCAAGAAGTATTATCATGATACTCTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG

pLck-3-2G6K: ATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGATTGTTAGCAGCAGCCAGTTAGCCTGGTACCAGCATAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGCTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGTAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTACCTCCCACGCTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC

I8-1B6 pHC-8-1B6H: ATGGACTGGACCTGGAGGGTCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCACAGGCGCAACTGGAGCAGTCTGGGGCTGAGGTGAGGAGGCCTGGGTCCTCGGTGAAGGTCGCCTGCAAGACTTCTGGAGGCATCTTCAGTAATTTTGCTGTCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATTCTCTCTATCTTTCGTACAACAAACTACGCACAGAAATTCCAGGGCAGAGTCACGATTACCGCGGACGAATCCACGAGCACAGCCTACATGGAGCTGAACAGCCTGAGATCTGACGACACGGCCGTCTATTACTGTGCGAGAAGCATTACAAATCTTTACTACTATTACATGGACGTCTGGGGCAAGGGGACCACGGTCACCGTCTCCTCAG

pLcL-8-1B6L: ATGCCCTGGGCTCTGCTCTTCCTCACCCTCCTTACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCGTCTCCTGCACTGGAACCAACAGTGACGTTGGTACTTATAACTATGTCTCCTGGTTCCAGCAGCACCCAGGCGAAGCCCCCAAAGTCATAATTTTTGATGTCAGTCATCGGCCCTCAGGGGTTTCTAACCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGACTGAGGACGAAGCTGATTATTACTGCAGCTCATATACAACCAGCAACACTCGAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAA

V3-3D2 pHC-3-3D2H: ATGGACTGGACCTGGAGGGTCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTGCAGCTGGTCCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGTAAGGCTCCTGGAGTCATCTTCAATGCCTATGCTATGAGCTGGGTGCGACAGGCCCCCGGACAAGGGCTTGAGTGGATGGGAGGGATCACCGGTGTCTTTCACACAGCAACCTACGCACCGAAGTTCCAGGGCAGAGTCACGATTACCGCGGACGAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGACGACACGGCCGTGTATTACTGTGCGCGAGGACCCAAGTACTACCACTCCTACATGGACGTCTGGGGCGAAGGGACCACGGTCACCGTCTCCTCAG

pLck-3-3D2K: ATGGACATGAGAGCCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCTTCACTGTCTGCATCTGTAGGAGATAGAGTCACCATCACTTGTCGGGCGAGTCAGGACATTAGCAATTATGTAGCCTGGTTTCAACAGAAACCAGGGAAAACCCCTAAGTCCCTGATGTATGCTACATCCAAATTGCAAAATGGGGTCCCTTCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAACTTATTACTGCCAACAGTATAGTCGTTATCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAAC

V3-1G10 pHC-3-1G10H: ATGGACTGGACCTGGAGCGTCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTGCAGTTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCCGGGTCCACGGTGAAGGTCTCCTGCGAGGCTTCTGGAGTCACCTTCAATCACTATACTGTCAGCTGGGTGCGACAAGCCCCTGGACAAGGACTTGAATGGATGGGAGGGATCATCCCTCTCTTTGGTACAGCAGACTACGCACAGAAGTTCCAGGACAGAGTCACAATTACCGCGGACAGATCCACGGGCACAGCCTACATGGAGCTGAGCTCCCTGAGACCTGAAGACACGGCCCTGTATTACTGTGCGAGATCCGGAACTACGAAGACGAGATATAACTGGTTCGACCCCTGGGGCCAGGGAACCACGGTCACCGTCTCCTCAG

pLck-3-1G10K: ATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACTCTTGGAGAAATAATAATGACGCAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCACCAACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGAGACAGAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCACTATAATAACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC

I5-24 pHC-5-24H: ATGGACTGGACCTGGAGCATCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCCCAATTCCAGTTGGTGCAATCTGGGGCTGAAGTGAGGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCACGGCCTCTGGAGGCACCTTCAGTAGATACACTGTCAACTGGGTGCGACAGGCCCCTGGACAGGGACTTCAGTGGATGGGCAGGTTCATCCCTCTCCTTGGTATGACAAACTACGCACAGAGGTTCCAGGGCAGAGCCACGATCACCGCGGACAAATCCACGACCACAGCCTTCTTGGAGCTGAGCAGCCTGACATCTGAGGACACGGCCGTCTATTTCTGTGCGAGACATGATAGCAGTGGTTATCACCCTCTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG

pLck-5-24K: ATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGCAGCGGCCACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCGGTGTATTACTGTCAGCAATATGCTGTCTTTCTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC

I4-128 pHC-4-128H: ATGGACTGGACCTGGAGGGTCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGATGGTCTCCTGCAAGGCCTCTGGAGGCACCTTCAGCACTTATGGTGTCAGCTGGGTGCGACAGGCCCCTGGACAAGGACTTGAGTGGGTGGGAGGAATCATCCCTATCTTTGGTACAGCAAAATACGCACAGAAGTTCCAGGGCAGAGTCACGATTACCGCGGACGAATCCTCGACCACAGCCTACATGGAGCTGAGCCGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGGCCTAACACCTATGGTTACATACTGCCCGTCTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG

pLck-4-128K: ATGGACATGAGGGCCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCGGTTGCCGGGCCAGTCAGACTATCAGTACCTACTTGGCCTGGTATCAGCAGGTGCCAGGGAAAGCCCCTAAACTCCTAATCTATATGGCGTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGGTGATTTTGCAACTTATTACTGCCAACATTATAACACTTATTCTTCTACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC

V4-17 pHC-4-17H: ATGAAGCACCTGTGGTTCTTCCTTCTGCTGGTGGCGGCTCCCAGATGGGTCCTGTCCCAGTTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCACTAGGAATAGTTACTTCTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGAGTATGTATTATGATGGGACCACCTACCACAACCCGTCCCTCAAGAGTCGACTCACCTTATCCGCGGACACGTCCAAGAACCAGTTCTCTGTGAGGCTGAGCTCTGTGACCGCCGCAGACACGGCTGTCTATTACTGTGCGAGACATCATGTTACGGAGTTACGAGTTTTGGAGTGGTTACCTAAGTCTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG

pLcL-4-17L: ATGGCCTGGATCGCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAACTTATTATGTACACTGGTACCAACACCTCCCAGGAACGGCCCCCAAACTCCTCATCTATGATAATAATCAGCGGCCGTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACTTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTACCACTGTGCAGCATGGGACGACAGCCTGAGTGGGGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAA

V3-2C3 pHC-3-2C3H: ATGGACTGGACCTGGAGGGTCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAACAACTATGCTGTCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGGTACAGCAAACTACGCACATAAGTTCCAGGGCAGAGTCACGATTACCGTGGACGAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCATGTATTACTGTGCGAGAGTTTGTAGTTTCTATGGTTCGGGGAGTTATTATAACGTGTTCTGCTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG

pLck-3-2C3K: ATGGACATGAGAGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGCAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCGGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCATCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACACTATAATAGTTATTCTCAGACTTTTGGCCAGGGGACCAAAGTGGAGATCAAAC

Listed are the nucleotide sequences of the variable region, including the signaling sequence that were cloned into the appropriate expression vectors.