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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Biochemistry. 2012 Apr 19;51(17):3565–3575. doi: 10.1021/bi300303g

Figure 6. The effect of various lipid head groups on γ-secretase activity.

Figure 6

Activity assays using purified γ-secretase and C100-FLAG substrate were performed in detergent-free vesicles comprising different ratios of various lipid types (A, B) mixed with whole brain PC extract at a total lipid concentration of 1.5 mM. Activity assay samples were analyzed by the same method as in Figure 2B-C. C) Aβ40 and Aβ42 ELISA data for whole brain lipid extract is given on a separate graph due to the different y-axis scale required. Statistical analyses were performed as in Figure 2 (n = 3-6).