Skip to main content
. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Biochemistry. 2012 Apr 19;51(17):3565–3575. doi: 10.1021/bi300303g

Figure 8. The effect of FA chain length and saturation on γ-secretase activity in living cells.

Figure 8

S20 CHO cells were treated with 50 μM lipid vesicles in standard growth media for 48 hr. Conditioned media were analyzed by 4G8 triplex Aβ ELISA (Mesoscale Discovery) and values normalized to the BCA assays of the cell lysates. A) Aβ levels are given as a percentage of normalized Aβ readings from vehicle-treated controls from the same plate. B) Aβ42/40 ratio is calculated from non-normalized readings, then given as a percentage of vehicle-treated controls from the same plate. Statistical analysis was performed as in Figure 2 (n = 5-12).